Contact an Account ManagerEnantiomeric separation of underivatized a-methyl-a-amino acids by HPLC
Summary
Type: HPLC
Application #: 105310
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: post-column derivatization: 10m PTFE loop (0.3mm ID), flow rate of the OPA reagent 0.9ml/min; detection: fluorescence, excitation 340nm, emission 425nm
Conditions: eluent: 2.5ml 0.32M DMV solution plus 2.5ml 0.16M copper(II) acetate solution diluted to 100ml (for some of the amino acids acetonitrile was added to decrease retention)
flow rate 0.3ml/min
Authors: Weinstein, S. et al.
Source: J. Chromatogr. 318 (1985) 117–121
Details
Legend: Fig. 1. Separation of a-Me-AA using as chiral additive the copper complex of
DMV dissolved in 0.1M Na acetate [Cu]:[DMV] = 8:16 (mM/mM), pH = 5.5.